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FARP1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • FC - FARP1 Polyclonal Antibody AP55071
    Blank control: Hela.
    Primary Antibody (green line): Rabbit Anti-FARP1 antibody (bs-13144R)
    Dilution: 1 µg /10^6 cells;
    Isotype Control Antibody (orange line): Rabbit IgG .
    Secondary Antibody : Goat anti-rabbit IgG-PE
    Dilution: 3 µg /test.
    Protocol
    The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 20% PBST for 20 min atroom temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
    detail
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immuno electron microscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P, IHC-F, IF, ICC, E
Primary Accession Q9Y4F1
Reactivity Rat, Pig, Dog, Bovine
Host Rabbit
Clonality Polyclonal
Calculated MW 118633 Da
Additional Information
Gene ID 10160
Other Names FERM, ARHGEF and pleckstrin domain-containing protein 1, Chondrocyte-derived ezrin-like protein, FERM, RhoGEF and pleckstrin domain-containing protein 1, Pleckstrin homology domain-containing family C member 2, PH domain-containing family C member 2, FARP1, CDEP, PLEKHC2
Dilution WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC=1:100-500,IF=1:100-500,Flow-Cyt=1ug/test,ELISA=1:5000-10000
Format0.01M TBS(pH7.4), 0.09% (W/V) sodium azide and 50% Glyce
StorageStore at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ℃.
Protein Information
Name FARP1
Synonyms CDEP, PLEKHC2
Function Functions as a guanine nucleotide exchange factor for RAC1. May play a role in semaphorin signaling. Plays a role in the assembly and disassembly of dendritic filopodia, the formation of dendritic spines, regulation of dendrite length and ultimately the formation of synapses (By similarity).
Cellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side. Synapse. Synapse, synaptosome Cytoplasm, cytosol. Cell projection, filopodium. Cell projection, dendrite. Cell projection, dendritic spine. Note=Recruited to the cell membrane via interaction with CADM1.
Tissue Location Detected in cAMP-treated chondrocytes, but not in untreated chondrocytes. Detected in fetal brain, heart and spleen, and in adult testis, kidney and lung.
Research Areas
Citations (0)
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$ 385.00
Cat# AP55071
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